@article { ISI:000268795100001,
	title = {Acute and subacute toxicity studies of chitosan reduced gold nanoparticles: a novel carrier for therapeutic agents},
	journal = {Journal of Biomedical Nanotechnology},
	volume = {5},
	number = {3},
	year = {2009},
	month = {JUN},
	pages = {233-239},
	publisher = {AMER SCIENTIFIC PUBLISHERS},
	address = {25650 NORTH LEWIS WAY, STEVENSON RANCH, CA 91381-1439 USA},
	abstract = {<p>The objective of the present study was to evaluate the oral toxicity of chitosan reduced gold nanoparticles so as to demonstrate its applicability for drug delivery application. Acute oral toxicity studies in female rats documented no deaths or treatment related complications. The LD(50) value of gold nanoparticles was found to be greater than 2000 mg/kg. In case of sub-acute oral toxicity studies, gold nanoparticles were administered orally to male and female rats for a period of 28-days. At the end of study blood samples were collected for haematology and biochemical analysis. For histopathological analysis, organs of animals were weighed and processed for examination. All animals survived the duration of the study, with no significant changes in clinical signs, body weight, food consumption, hematological parameters, organ weights and histopathological findings. These studies establish that chitosan reduced gold nanoparticles produced no treatment related toxicity in rats following oral administration, thus can be exploited for potential therapeutic applications.</p>},
	keywords = {Acute Toxicity, Chitosan, gold nanoparticles, Sub-Acute Toxicity, Wistar Rats},
	issn = {1550-7033},
	doi = {10.1166/jbn.2009.1027},
	author = {Pokharkar, Varsha and Dhar, Sheetal and Bhumkar, Devika and Mali, Vishal and Bodhankar, Subhash L. and Bhagavatula L. V. Prasad}
}
@article { ISI:000266197300018,
	title = {Hypocholesteremic effect of bile salt hydrolase from Lactobacillus buchneri ATCC 4005},
	journal = {Food Research International},
	volume = {42},
	number = {4},
	year = {2009},
	note = {International Congress on Bioprocesses in Food Industries, Hyderabad, INDIA, NOV 06-08, 2008},
	month = {MAY},
	pages = {516-520},
	publisher = {ICBF Forum; Biotech Res Soc India},
	address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
	abstract = {<p>The effect of oral administration of the immobilized bile salt hydrolase enzyme on serum cholesterol. triglyceride, high density lipoprotein levels and its application in the therapeutic treatment of hypercholesteremia was assessed. Culture conditions were optimized for the production of bile salt hydrolase, which resulted in 2.9-fold enhancement in activity. Bile salt hydrolase (BSH; E.C.3.5.1.24) was isolated from Lactobacillus buchneri ATCC 4005 and immobilized in 0.5\% gellan gum gel. The immobilized enzyme was orally delivered in wistar rats, induced with hypercholesteremia by triton X-100. The serum cholesterol and triglycerides were reduced by 50\% and 15\%, respectively, in the group fed with immobilized enzyme 10 IU/kg dose whereas administration of 20 IU/kg immobilized enzyme resulted in reduction of serum cholesterol by 58\% and triglycerides by 45\%, respectively. The results indicate that bile salt hydrolase has potential cholesterol lowering property and oral administration of the immobilized enzyme is an alternative pharmacological approach to reduce serum cholesterol levels. (C) 2009 Elsevier Ltd. All rights reserved.</p>},
	keywords = {Bile salt hydrolase, Gellan gum, Hypocholesteremic effect, Lactobacillus buchneri, Wistar Rats},
	issn = {0963-9969},
	doi = {10.1016/j.foodres.2009.02.016},
	author = {Sridevi, N. and Vishwe, Pradnya and Prabhune, Asmita}
}
