@article {48108,
	title = {2,4-Di-tert-butylphenol (2,4-DTBP) purified from streptomyces sp. KCA1 from phyllanthus niruri: isolation, characterization, antibacterial and anticancer properties},
	journal = {Journal of King Saud University Science},
	volume = {34},
	year = {2022},
	month = {JUL},
	pages = {102088},
	type = {Article},
	abstract = {<p>
	Objectives: The present study reports the bioactivity of 2,4-Di-tert-butylphenol (2,4-DTBP) purified from an endophytic Streptomyces species KCA1 isolated from the leaves of Phyllanthus niruri and its antibacterial and anticancer properties. Methods: The extracellular metabolites were produced from the strain KCA1 by submerged fermentation using ethyl acetate. The crude extract was further evaluated for antibacterial activity against set of pathogens. Active metabolite from the extract was purified using chromatography techniques and detected its antibacterial activity by disc diffusion assay. The molecular structure of the active molecule was identified through various spectral study. Moreover, the bioactive metabolite 2,4-DTBP was analyzed antibacterial and anti-proliferative activity. Results: Strain KCA1 was identified as Streptomyces sp. In the preliminary screening, the crude extract exhibited broad spectrum activity against various bacterial pathogens. Based on the spectral properties, the active metabolite was identified as 2,4-Di-tert-butylphenol. The MIC of active compound 2,4-DTBP inhibited E. coli ATCC 25922 and S. aureus ATCC 29213 at 50 lg/ml and 0.78 lg/ml, respectively. The IC50 value of 2,4-DTBP was found to be 11.0 lg/ml and 116.8 lg/ml, against breast cancer cell line (MCF7) and normal VERO cell line, respectively. Conclusions: This study concluded that 2,4-DTBP, produced from the endophytic Streptomyces sp. KCA1, is the potential candidate to develop as promising antibacterial and anticancer agent. (C) 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University.</p>
},
	keywords = {2, 4-Di-tert-butylphenol, Antibacterial, antiproliferative activity, Endophytic Streptomyces},
	issn = {1018-3647},
	doi = {10.1016/j.jksus.2022.102088},
	author = {Seenivasan, Ayswarya and Manikkam, Radhakrishnan and Kaari, Manigundan and Sahu, Amit Kumar and Said, Madhukar and Dastager, Syed G.}
}
@article {48014,
	title = {Antifungal compound from marine Serratia marcescens BKACT and its potential activity against Fusarium sp.},
	journal = {International Microbiology},
	volume = {25},
	year = {2022},
	month = {NOV},
	pages = {851-862},
	type = {Article},
	abstract = {<p>
	Ecofriendly biocontrol agents to control pathogenic fungi are in demand globally. The present study evaluated the antifungal potentials of marine bacteria Serratia marcescens BKACT against eight different Fusarium species. A highest 75.5 +/- 0.80\% of mycelial inhibition was observed against Fusarium foetens NCIM 1330. Structural characterization of the purified compound was analyzed by GC-MS and NMR techniques; based on the analysis, it is confirmed as 2, 4-di-tert butyl phenol (2, 4-DTBP) with chemical structure C14H22O. At 0.53 mM concentration, purified compound inhibited complete spore germination of F. foetens NCIM 1330. In vitro assay showed complete inhibition of F. foetens NCIM 1330 on the wheat seeds. Tested concentration does not show any toxic effect on germination of the seeds. By this study, we conclude that, 2, 4-DTBP is a suitable candidate to be used as biocontrol agent against Fusarium infection.</p>
},
	keywords = {2, 4, di-tert butyl phenol, Fusarium sp, marine, Serratia marcescens},
	issn = {1139-6709},
	doi = {10.1007/s10123-022-00268-3},
	author = {Kharat, Bharat A. and Said, Madhukar S. and Dastager, Syed G.}
}
