@article { ISI:000245010200010, title = {Differentially expressed gene transcripts in roots of resistant and susceptible chickpea plant (Cicer arietinum L.) upon Fusarium oxysporum infection}, journal = {Physiological and Molecular Plant Pathology}, volume = {68}, number = {4-6}, year = {2006}, month = {APR}, pages = {176-188}, publisher = {ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD}, type = {Article}, address = {24-28 OVAL RD, LONDON NW1 7DX, ENGLAND}, abstract = {

Differentially expressed genes in chickpea, (Cicer arietinum L.) during root infection by Fusarium oxysportan f sp. ciceri Racel, were identified using cDNA-RAPD and cDNA-AFLP approaches. The former employed decamer primers on cDNA template and revealed nine differentially expressed transcripts in the resistant-infected chickpea variety. Among the 2000 transcript-derived fragments (TDFs) screened by cDNA-AFLP, 273 were differentially expressed in chickpea roots during Fusaritan infection. Only 13.65\% of the TDFs were differentially regulated during pathogen challenge, while the other 86\% were expressed non-differentially during the process of pathogen infection in chickpea roots. Nineteen TDFs, which expressed differentially in the resistant-infected chickpea variety were cloned and sequenced. Two of these TDFs were similar to transcription factors like WRKY proteins and 14-3-3 proteins, while three TDFs represented the NBS-LRR-type gene sequences. Two TDFs had sequence identity to genes known to have function in defense. The RAPID TDF CaFRi60 showed sequence identity to gamma-glutamyl-cysteine synthetase. Among the TDFs examined by cDNA-AFLP, 19 were confirmed by Reverse Northern blot to be differentially expressed. The data confirms the effectiveness of the cDNA-AFLP technique in detecting differentially expressed genes during pathogenesis. (c) 2006 Elsevier Ltd. All rights reserved.

}, keywords = {cDNA-AFLP, Chickpea, Cicer arietinum, differentially expressed genes, fusarium oxysporum, Transcriptome}, issn = {0885-5765}, doi = {10.1016/j.pmpp.2006.10.003}, author = {Nimbalkar, Suhas B. and Harsulkar, Abhay M. and Giri, Ashok P. and Sainani, Mohini N. and Franceschi, Vincent R. and Gupta, Vidya S.} } @article { ISI:000269059100008, title = {Molecular mapping of wilt resistance genes in chickpea}, journal = {Molecular Breeding}, volume = {24}, number = {2}, year = {2009}, month = {SEP}, pages = {177-183}, publisher = {SPRINGER}, address = {VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS}, abstract = {

Fusarium wilt is a widespread and serious chickpea disease caused by the soil-borne fungus Fusarium oxysporum f.sp. ciceri (Foc). We evaluated an F(9) recombinant inbred line population of chickpea for resistance to three Foc races (1, 2 and 3) in pot culture experiments and identified flanking and tightly linked DNA markers for the resistance genes. The simple sequence repeat markers H3A12 and TA110 flanked the Foc1 locus at 3.9 and 2.1 cM, respectively, while Foc2 was mapped 0.2 cM from TA96 and 2.7 cM from H3A12. The H1B06y and TA194 markers flanked the Foc3 locus at 0.2 and 0.7 cM, respectively. These markers were also validated using 16 diverse chickpea genotypes. Identification of tightly linked flanking markers for wilt resistance genes will be useful for their exploitation in breeding programs and to understand the mechanism of resistance and evolution of the genes.

}, keywords = {fusarium oxysporum, Fusarium wilt, Genetic mapping, Marker validation, Pathogen races}, issn = {1380-3743}, doi = {10.1007/s11032-009-9282-y}, author = {Gowda, S. J. M. and Radhika, P. and Kadoo, Narendra Y. and Mhase, L. B. and Gupta, V. S.} } @article { ISI:000317939100003, title = {Biotransformation of vinblastine to vincristine by the endophytic fungus fusarium oxysporum isolated from catharanthus roseus}, journal = {Biocatalysis and Biotransformation}, volume = {31}, number = {2}, year = {2013}, month = {APR}, pages = {89-93}, publisher = {INFORMA HEALTHCARE}, address = {TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND}, abstract = {

A fungal transformation method for the production of the less abundant but more valuable anticancer drug vincristine using the more abundant anticancer drug vinblastine by an endophytic fungus Fusarium oxysporum isolated from the plant Catharanthus roseus is described. Vinblastine dissolved in sterile water when incubated with the fungal mycelial mass was transformed into vincristine at room temperature. The biotransformation process was monitored by periodic sampling of aliquots that were extracted with ethyl acetate. The crude transformed compounds were analysed using TLC where the Rf value of the spot obtained on the 8th day was found to be similar to that of standard vincristine (Rf 0.74). Purification by HPLC using a C 18 symmetry column showed a peak with RT (34.9 min) similar to that of standard vincristine. Further characterization with the help of ESI-MS showed a peak at m/z 825 attributed to the (M + H)(+) ion. This indicated that the fungus Fusarium oxysporum had converted vinblastine to vincristine.

}, keywords = {Biotransformation, endophytes, fusarium oxysporum, Vinblastine, vincristine}, issn = {1024-2422}, doi = {10.3109/10242422.2013.776544}, author = {Kumar, Ashutosh and Ahmad, Absar} } @article { ISI:000380072400017, title = {Biogenic terbium oxide nanoparticles as the vanguard against osteosarcoma}, journal = {Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy}, volume = {168}, year = {2016}, month = {NOV}, pages = {123-131}, publisher = {PERGAMON-ELSEVIER SCIENCE LTD}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND}, abstract = {

The synthesis of inner transition metal nanoparticles via an ecofriendly route is quite difficult. This study, for the first time, reports synthesis of terbium oxide nanoparticles using fungus, Fusarium oxysporum. The biocompatible terbium oxide nanoparticles (Tb2O3 NPs) were synthesized by incubating Tb4O7 with the biomass of fungus F. oxysporum. Multiple physical characterization techniques, such as UV-visible and photoluminescence spectroscopy, TEM, SAED, and zeta-potential were used to confirm the synthesis, purity, optical and surface characteristics, crystallinity, size, shape, distribution, and stability of the nanoemulsion of Tb2O3 NPs. The Tb2O3 NPs were found to inhibit the propagation of MG-63 and Saos-2 cell-lines (IC50 value of 0.102 mu g/mL) and remained non-toxic up to a concentration of 0373 gg/mL toward primary osteoblasts. Cell viability decreased in a concentration-dependent manner upon exposure to 10 nmTb(2)O(3) NPs in the concentration range 0.023-0373 mu g/mL Cell toxicity was evaluated by observing changes in cell morphology, cell viability, oxidative stress parameters, and FACS analysis. Morphological examinations of cells revealed cell shrinkage, nuclear condensation, and formation of apoptotic bodies. The level of ROS within the cells an indicator of oxidative stress was significantly increased. The induction of apoptosis at concentrations \<= IC50 was corroborated by 4{\textquoteright},6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (DNA damage and nuclear fragmentation). Flow-cytometric studies indicated that the response was dose dependent with a threshold effect. (C) 2016 Elsevier B.V. All rights reserved.

}, keywords = {Biosynthesis, Cytotoxicity, fusarium oxysporum, Osteosarcoma, Tb2O3 nanoparticles}, issn = {1386-1425}, doi = {10.1016/j.saa.2016.05.053}, author = {Iram, Sana and Khan, Salman and Ansary, Abu Ayoobul and Arshad, Mohd and Siddiqui, Sahabjada and Ahmad, Ejaz and Khan, Rizwan H. and Khan, Mohd Sajid} } @article { ISI:000378741200010, title = {Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics}, journal = {Plant Biotechnology Journal}, volume = {14}, number = {7}, year = {2016}, month = {JUL}, pages = {1589-1603}, publisher = {WILEY-BLACKWELL}, address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA}, abstract = {

Molecular changes elicited by plants in response to fungal attack and how this affects plant-pathogen interaction, including susceptibility or resistance, remain elusive. We studied the dynamics in root metabolism during compatible and incompatible interactions between chickpea and Fusarium oxysporum f. sp. ciceri (Foc), using quantitative label-free proteomics and NMR-based metabolomics. Results demonstrated differential expression of proteins and metabolites upon Foc inoculations in the resistant plants compared with the susceptible ones. Additionally, expression analysis of candidate genes supported the proteomic and metabolic variations in the chickpea roots upon Foc inoculation. In particular, we found that the resistant plants revealed significant increase in the carbon and nitrogen metabolism; generation of reactive oxygen species (ROS), lignification and phytoalexins. The levels of some of the pathogenesis-related proteins were significantly higher upon Foc inoculation in the resistant plant. Interestingly, results also exhibited the crucial role of altered Yang cycle, which contributed in different methylation reactions and unfolded protein response in the chickpea roots against Foc. Overall, the observed modulations in the metabolic flux as outcome of several orchestrated molecular events are determinant of plant{\textquoteright}s role in chickpea-Foc interactions.

}, keywords = {Chickpea, fusarium oxysporum, Metabolomics, NMR, plant-pathogen interaction, proteomics}, issn = {1467-7644}, doi = {10.1111/pbi.12522}, author = {Kumar, Yashwant and Zhang, Limin and Panigrahi, Priyabrata and Dholakia, Bhushan B. and Dewangan, Veena and Chavan, Sachin G. and Kunjir, Shrikant M. and Wu, Xiangyu and Li, Ning and Rajmohanan, Pattuparambil R. and Kadoo, Narendra Y. and Giri, Ashok P. and Tang, Huiru and Gupta, Vidya S.} }