@article { ISI:000320024400001,
	title = {C-C Bond formation catalyzed by natural gelatin and collagen proteins},
	journal = {Beilstein Journal of Organic Chemistry},
	volume = {9},
	year = {2013},
	month = {JUN},
	pages = {1111-1118},
	publisher = {BEILSTEIN-INSTITUT},
	address = {TRAKEHNER STRASSE 7-9, FRANKFURT AM MAIN, 60487, GERMANY},
	abstract = {<p>The activity of gelatin and collagen proteins towards C-C bond formation via Henry (nitroaldol) reaction between aldehydes and nitroalkanes is demonstrated for the first time. Among other variables, protein source, physical state and chemical modification influence product yield and kinetics, affording the nitroaldol products in both aqueous and organic media under mild conditions. Significantly, the scale-up of the process between 4-nitrobenzaldehyde and nitromethane is successfully achieved at 1 g scale and in good yield. A comparative kinetic study with other biocatalysts shows an increase of the first-order rate constant in the order chitosan \&lt; gelatin \&lt; bovine serum albumin (BSA) \&lt; collagen. The results of this study indicate that simple edible gelatin can promote C-C bond forming reactions under physiological conditions, which may have important implications from a metabolic perspective.</p>},
	keywords = {biocatalysis, carbon-carbon bond formation, gelatin, Henry reaction, protein},
	issn = {1860-5397},
	doi = {10.3762/bjoc.9.123},
	author = {Kuehbeck, Dennis and Dhar, Basab Bijayi and Schoen, Eva-Maria and Cativiela, Carlos and Gotor-Fernandez, Vicente and Diaz, David Diaz}
}
@article {49581,
	title = {Low-cost gelatin/collagen scaffolds for bacterial growth in bioreactors for biotechnology},
	journal = {Applied Microbiology and Biotechnology},
	volume = {109},
	year = {2025},
	month = {MAY},
	pages = {113},
	type = {Article},
	abstract = {<p>
	A wide array of pharmaceutical and industrial products available in today{\textquoteright}s market stems from bioreactors. Meeting the escalating demand for these products necessitates significant enhancements in biotechnological processes. This study focuses on developing cost-effective scaffolds designed explicitly for use within bioreactors, employing commonly used polymers such as gelatin and collagen. Bacterial proliferation assays involving Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were conducted to assess the effectiveness of these scaffolds. The scaffolds were produced by electrospinning polymeric solutions with varying concentrations of gelatin and collagen and were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetric analysis. Results revealed that scaffolds with 15\% gelatin increased the 24-h proliferation of S. aureus, P. aeruginosa, and E. coli by 52\%, 35\%, and 20\%, respectively. In the case of E. coli, scaffolds with lower gelatin concentrations (1-10\%) were more effective, leading to 35-55\% proliferation growth. These findings highlight the potential application of gelatin/collagen scaffolds in fabricating industrial products derived from these bacteria.</p>
},
	keywords = {Bacterial cell proliferation, collagen, gelatin, Polymeric electrospinning, scaffolds},
	issn = {0175-7598},
	doi = {10.1007/s00253-025-13491-5},
	author = {Pompa-Monroy, Daniella Alejandra and Vera-Graziano, Ricardo and Dastager, Syed G. and Perez-Gonzalez, Graciela Lizeth and Bogdanchikova, Nina and Iglesias, Ana Leticia and Villarreal-Gomez, Luis Jesus}
}
