@article { ISI:000326292100005,
	title = {G-Quadruplexes as tools for synthetic biology},
	journal = {Chembiochem},
	volume = {14},
	number = {16},
	year = {2013},
	month = {NOV},
	pages = {2077-2081},
	publisher = {WILEY-V C H VERLAG GMBH},
	address = {BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY},
	abstract = {<p>With the potential to engineer biological systems, synthetic biology is an emerging field that combines various disciplines of sciences. It encompasses combinations of DNA, RNA and protein modules for constructing desired systems and the rewiring of existing signalling networks. Despite recent advances, this field still lags behind in the artificial reconstruction of cellular processes, and thus demands new modules and switches to create genetic circuits. The widely characterised noncanonical nucleic acid secondary structures, G-quadruplexes are promising candidates to be used as biological modules in synthetic biology. Structural plasticity and functional versatility are significant G-quadruplex traits for its integration into a biological system and for diverse applications in synthetic circuits.</p>},
	keywords = {G-quadruplexes, ribozymes, synthetic biology, topology, zipcodes},
	issn = {1439-4227},
	doi = {10.1002/cbic.201300456},
	author = {Agarwala, Prachi and Pandey, Satyaprakash and Maiti, Souvik}
}
@article {47123,
	title = {Metabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae},
	journal = {Applied Microbiology and Biotechnology},
	volume = {105},
	year = {2021},
	month = {JAN},
	pages = {457-475},
	type = {Review},
	abstract = {<p>Isoprenoids, often called terpenoids, are the most abundant and highly diverse family of natural organic compounds. In plants, they play a distinct role in the form of photosynthetic pigments, hormones, electron carrier, structural components of membrane, and defence. Many isoprenoids have useful applications in the pharmaceutical, nutraceutical, and chemical industries. They are synthesized by various isoprenoid synthase enzymes by several consecutive steps. Recent advancement in metabolic engineering and synthetic biology has enabled the production of these isoprenoids in the heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Both heterologous systems have been engineered for large-scale production of value-added isoprenoids. This review article will provide the detailed description of various approaches used for engineering of methyl-d-erythritol-4-phosphate (MEP) and mevalonate (MVA) pathway for synthesizing isoprene units (C-5) and ultimate production of diverse isoprenoids. The review particularly highlighted the efforts taken for the production of C-5-C-20 isoprenoids by metabolic engineering techniques in E. coli and S. cerevisiae over a decade. The challenges and strategies are also discussed in detail for scale-up and engineering of isoprenoids in the heterologous host systems.</p>
},
	keywords = {Escherichia coli, Isoprenoids, Metabolic engineering, Saccharomyces cerevisiae, synthetic biology},
	issn = {0175-7598},
	doi = {10.1007/s00253-020-11040-w, Early Access Date = JAN 2021},
	author = {Navale, Govinda R. and Dharne, Mahesh S. and Shinde, Sandip S.}
}
