@article {46816,
	title = {Combinatorial approach through in vitro regeneration and phytochemical profiling of ceropegia media (huber) ans.: a potential way forward in the conservation of an endangered medicinal plant from the western ghats in India},
	journal = {Journal of Plant Growth Regulation},
	year = {2020},
	month = {JUN },
	type = {Article; Early Access 2020},
	abstract = {<p>Ceropegia mediais an endemic and endangered plant as its propagation through seeds is unreliable due to low germination, slow growth and seedling decay under natural conditions. Also, tubers of this plant are edible serving as carbohydrate source with medicinal values leading to severe population decline in the natural habitat. To provide a sustainable solution, an efficient in vitro propagation protocol along with phytochemical profiling was developed forC. media. Callus cultures were induced from seedling and wild leaf tissues using the most effective Murashige and Skoog{\textquoteright}s (MS) medium with 2,4-dichlorophenoxyacetic acid (2,4-D; 2 mu M) and sucrose (3\%). Somatic embryos were acquired on MS medium with 1 mu M 6-Benzylaminopurine (BAP) and 1 mu M 2,4-D. Conversion into plantlets was attained only from tissue culture-derived seedling leaf (TCDSL) explant. Further, in vitro tuberization was achieved from TCDSL callus with BAP and Naphthalene acetic acid (NAA). AgNO(3)as an elicitor had a positive effect on both fresh and dry weights of callus. Successful acclimatization (58\%) was attained after two months resulting in normal phenotype in pots. Further, metabolite profiles of ten different tissues from wild and in vitro plants were compared. Total 82 compounds comprising alkaloids, fatty acids, fatty acid ester, steroids, terpenes and hydrocarbons were identified. Overall, results suggested enhanced production of selected metabolites with in vitro propagation and AgNO3, alleviating the problem of unavailability of planting materials. Thus, the current study might offer potential ways for the conservation of such RED enlisted species asC. media. [GRAHICS]</p>
},
	keywords = {Ceropegia media, GC-MS, Microtuberization, Secondary metabolites, Silver nitrate, somatic embryogenesis},
	issn = {0721-7595},
	doi = {10.1007/s00344-020-10173-6, Early Access Date = JUN 2020},
	author = {Pandey, Meena and Dholakia, Bhushan B. and Jayaramaiah, Ramesha H. and Punekar, Sachin A. and Giri, Ashok P.}
}
@article {47316,
	title = {Synthesis of gold and silver nanoparticles using Phlogacanthus Thyrsiformis extracts-its phytochemical screening and anti-oxidant studies},
	journal = {Materials Today-Proceedings},
	volume = {43},
	year = {2021},
	month = {JAN},
	pages = {2805-2809},
	type = {Proceedings Paper},
	abstract = {<p>Phlogacanthus Thyrsiformis is a shrub belonging to the Acanthaceae family, normally found in the northeastern parts of India, Nepal, Burma, Indo-Chinese region and Indonesia. It is used as a cure for diarrhoea, dysentery, cholera, burns, boils, high blood pressure, sprains and body pains. Silver and gold nanoparticles were synthesized from the extracts of the leaves of this plant as the some biomolecules, known as secondary metabolites, present in plant extracts can be used to reduce metal ions to nanoparticles. The contents of the leaves were extracted in various solvents and the tests for the presence of secondary metabolites were carried out. The gold and silver nanoparticles synthesized using the plant extracts and characterized using TEM, SEM techniques and the phytochemical screening tests were done for different extracts. The anti-oxidant studies of the several extracts were carried out. (C) 2020 Elsevier Ltd. All rights reserved.</p>},
	keywords = {Anti-oxidant studies, Nanoparticles, Phligacanthus thyrsiformis, Phytochemical Studies, Secondary metabolites},
	issn = {2214-7853},
	doi = {10.1016/j.matpr.2020.08.798},
	author = {Singh, Avinash Thounaojam and Shaikh, Iqbal N. and Rale, Madhura Vijay}
}
