@article { ISI:000346371700012,
	title = {Evaluation of genetic variability in Symplocos laurina Wall. from two biodiversity hotspots of India},
	journal = {Plant Systematics and Evolution},
	volume = {300},
	number = {10},
	year = {2014},
	month = {DEC},
	pages = {2239-2247},
	publisher = {SPRINGER WIEN},
	address = {SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA},
	abstract = {<p>Symplocos laurina Wall., an important medicinal tree species, is one of the pioneering plants for establishment of shola forest ecosystem. We analyzed 208 accessions of S. laurina from 13 populations belonging to three ecoregions in India namely, Western Ghats, Eastern Ghats and Northeast India, to comment on the genetic diversity and population structure of the species. Of the 100 inter simple sequence repeat primers screened, eight were selected based on their clear and reproducible polymorphic patterns. These eight ISSR primers produced on an average 1,014 bands from 208 accessions, of which 6.58 \% were rare bands, 55.96 \% were shared bands and 37.46 \% were similar bands. The primers, UBC835 and UBC880, amplified the highest number of polymorphic loci (16), while the primer UBC852 amplified the least number of amplicons (9). The values for total genetic diversity (H-T) and the average heterozygosity (H-S) were 0.3407 and 0.2263, respectively; while the coefficient of genetic differentiation (G(ST)) was 0.3358. Within-population variance was higher (68 \%) than among-population variance (17 \%), indicating high intra-population genetic diversity. These populations were grouped according to their geographical location in cluster analysis. Mantel test also showed significant positive correlation between genetic and geographical distances. The present study revealed the importance of the Palghat gap in South India in shaping the distribution of genetic diversity in S. laurina.</p>},
	keywords = {Biodiversity hotspots, Genetic diversity, ISSR, Palghat gap, Symplocos laurina},
	issn = {0378-2697},
	doi = {10.1007/s00606-014-1046-4},
	author = {Bhagwat, Rasika M. and Banu, Sofia and Dholakia, Bhushan B. and Kadoo, Narendra Y. and Lagu, Meena D. and Gupta, Vidya S.}
}
@article { ISI:000358761200004,
	title = {Expression of stable reference genes and SPINDLY gene in response to gibberellic acid application at different stages of grapevine development},
	journal = {Biologia Plantarum},
	volume = {59},
	number = {3},
	year = {2015},
	month = {SEP},
	pages = {436-444},
	publisher = {ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY},
	address = {ROZVOJOVA 263, PRAHA 6, CZ-165 02, CZECH REPUBLIC},
	abstract = {<p>Gibberellic acid (GA(3)) is widely used at different stages of berry development, and to understand the molecular mechanism of its action requires identification of stable reference genes. We sprayed grapevine (Vitis vinifera L.) cv. Thompson Seedless with GA(3) at rachis stage for rachis elongation, at flower cluster stage for flower thinning, and at 3 - 4 mm berry stage for berry elongation. Tissue samples were collected at different time points after GA(3) application. The expression of 10 candidate reference genes was analyzed using 4 different algorithms to assess their suitability for real time-PCR data normalization. Based on the overall ranking, PP2A, Sutra, and SAND were identified as the most stably expressed genes across all samples. With regard to different stages, tubulin, EF1 alpha, and UBC were the most stable genes during rachis elongation; PP2A, SAND, and Sutra were the most suitable at the flower cluster and berry stages. The expression of GA signaling gene SPINDLY (VvSpy) was analyzed to validate the stable reference genes. After the GA(3) application, the expression of VvSpy was reduced at the rachis stage but did not change at the flower cluster and berry stages. The expression profile of VvSpy was comparable when two or three reference genes were used for data normalization.</p>},
	keywords = {developmental stages, Vitis vinifera},
	issn = {0006-3134},
	doi = {10.1007/s10535-015-0521-2},
	author = {Upadhyay, A. and Jogaiah, S. and Maske, S. R. and Kadoo, Narendra Y. and Gupta, V. S.}
}
