@article {48712,
	title = {Permeable polymersomes from temperature and pH dual stimuli-responsive PVCL-b-PLL block copolymers for enhanced cell internalization and lysosome targeting},
	journal = {Biomaterials Advances},
	volume = {151},
	year = {2023},
	month = {AUG},
	pages = {213454},
	type = {Article},
	abstract = {<p>
	A series of dual stimuli-responsive block copolymers comprising temperature-responsive poly(N-vinyl-caprolactam) (PVCL) and biodegradable pH-responsive poly(L-lysine) (PLL) of varying chain length were syn-thesized by a combination of free radical polymerization and ring opening polymerization. The block copolymers formed micelles and vesicles (polymersomes) in response to temperature and pH, respectively, in aqueous so-lution. The nanoassemblies were characterized by transmission electron microscopy and dynamic light scattering techniques. Encapsulation of both hydrophobic and hydrophilic dyes in the polymersomes was shown. Doxo-rubicin (DOX) was loaded in the polymersomes and its controlled release in response to the two stimuli, inde-pendently and jointly, was studied. The drug was found to be released due to stimuli-induced increased permeability without disassembly of the polymersomes. A significant increase in the cellular uptake of the drug-loaded polymersomes at hyperthermia conditions was demonstrated at 41 degrees C and release of the drug upon localization in lysosomes was observed. Cellular internalization pathway of the polymersomes was investigated by competitive inhibition assay and a combination of endocytic pathways dominated by caveolae-mediated mechanism was found to be operative.</p>
},
	keywords = {block copolymers, Controlled release, drug delivery, endocytosis, Polymersomes, Stimuli-responsive},
	doi = {10.1016/j.bioadv.2023.213454},
	author = {Surapaneni, Sai Geetika and Choudhari, Shakeb N. and Avhad, Shankarrao V. and Ambade, Ashootosh V.}
}
@article {49543,
	title = {Visible light-responsive composition-dependent morphology and cargo release in mixed micelles of dendron amphiphiles},
	journal = {Langmuir},
	volume = {41},
	year = {2025},
	month = {JAN},
	pages = {1957-1967},
	type = {Article},
	abstract = {<p>
	2,2-Bis-(methylol)propionic acid-based second-generation polyester dendron amphiphile (T-D) containing visible light-responsive donor-acceptor Stenhouse adduct (DASA) as hydrophobic tails is synthesized. Micelles of T-D amphiphile and its mixed micelles of varying compositions with nonresponsive dendron amphiphile containing lauryl groups are prepared in aqueous solution. In transmission electron microscopy and atomic force microscopy analyses, T-D amphiphiles show rice grain-like ellipsoidal micelles as the predominant morphology. Mixed micelles display a composition-dependent morphology gradient such that the morphology changes from rice grain like to mixed to completely spherical with decreasing content of the T-D amphiphile. Complete morphology change to spherical micelles and partial reversal to ellipsoidal micelles, finally leading to ill-defined aggregates, are observed when the T-D amphiphile micelles are subjected to visible light-dark storage photoswitching cycles. Small-angle neutron scattering (SANS) analysis of 1 wt.\% micellar solution in THF:water (10:90) reveals only a minor change in shape and size upon photoirradiation, and the data could be fitted to spherical or ellipsoidal model. Release of hydrophobic dye from mixed micelles is tuned by the content of the photoresponsive amphiphile. Cellular uptake and visible light-triggered release of hydrophobic drug from mixed micelles are demonstrated using MDA-MB-231 cells, suggesting their applicability for photoresponsive drug delivery.</p>
},
	issn = {0743-7463},
	doi = {10.1021/acs.langmuir.4c04509},
	author = {Avhad, Shankarrao V. and Kumar, Sugam and Ambade, Ashootosh V.}
}
