@article {48039,
	title = {Poly(N-vinylcaprolactam) containing solid lipid polymer hybrid nanoparticles for controlled delivery of a hydrophilic drug gemcitabine hydrochloride},
	journal = {RSC Advances},
	volume = {12},
	year = {2022},
	month = {JUN},
	pages = {17621-17628},
	type = {Article},
	abstract = {<p>
	Folic acid tagged and hydrophilic polymer containing solid lipid nanoparticles (SLNs) were formulated for the controlled and targeted delivery of gemcitabine, a hydrophilic drug. Drug loaded SLNs were prepared by double emulsion method and optimized by 3(2) level factorial design. Then, a hydrophilic polymer, namely, poly(N-vinylcaprolactam) (PVCL) was incorporated in the optimized SLN batch in the first aqueous phase (W1) to obtain solid lipid polymer hybrid nanoparticles (SLPHNs) that were further decorated with folic acid (F-SLPHNs). TEM analysis of SLNs and SLPHNs revealed the spherical shape with no aggregation while SLPHNs showed higher \% EE. SLPHNs exhibited limited burst release of gemcitabine compared to SLNs as well as lower overall \% release. All the formulations showed good cytocompatibility against MDA-MB-231 cell lines and folic acid-tagged hybrid particles (F-SLPHNs) showed remarkably higher cellular uptake.</p>
},
	doi = {10.1039/d2ra02845j},
	author = {Surapaneni, Sai Geetika and Ambade, V. Ashootosh}
}
@article {48712,
	title = {Permeable polymersomes from temperature and pH dual stimuli-responsive PVCL-b-PLL block copolymers for enhanced cell internalization and lysosome targeting},
	journal = {Biomaterials Advances},
	volume = {151},
	year = {2023},
	month = {AUG},
	pages = {213454},
	type = {Article},
	abstract = {<p>
	A series of dual stimuli-responsive block copolymers comprising temperature-responsive poly(N-vinyl-caprolactam) (PVCL) and biodegradable pH-responsive poly(L-lysine) (PLL) of varying chain length were syn-thesized by a combination of free radical polymerization and ring opening polymerization. The block copolymers formed micelles and vesicles (polymersomes) in response to temperature and pH, respectively, in aqueous so-lution. The nanoassemblies were characterized by transmission electron microscopy and dynamic light scattering techniques. Encapsulation of both hydrophobic and hydrophilic dyes in the polymersomes was shown. Doxo-rubicin (DOX) was loaded in the polymersomes and its controlled release in response to the two stimuli, inde-pendently and jointly, was studied. The drug was found to be released due to stimuli-induced increased permeability without disassembly of the polymersomes. A significant increase in the cellular uptake of the drug-loaded polymersomes at hyperthermia conditions was demonstrated at 41 degrees C and release of the drug upon localization in lysosomes was observed. Cellular internalization pathway of the polymersomes was investigated by competitive inhibition assay and a combination of endocytic pathways dominated by caveolae-mediated mechanism was found to be operative.</p>
},
	keywords = {block copolymers, Controlled release, drug delivery, endocytosis, Polymersomes, Stimuli-responsive},
	doi = {10.1016/j.bioadv.2023.213454},
	author = {Surapaneni, Sai Geetika and Choudhari, Shakeb N. and Avhad, Shankarrao V. and Ambade, Ashootosh V.}
}
