@article {44357,
	title = {Favorable 2 {\textquoteright}-substitution in the loop region of a thrombin-binding DNA aptamer},
	journal = {Bioorganic \& Medicinal Chemistry Letters},
	volume = {28},
	year = {2018},
	month = {JUN},
	pages = {1765-1768},
	type = {Article},
	abstract = {Simple 2{\textquoteright}-OMe-chemical modification in the loop region of the 15mer G-rich DNA sequence GGTTGGTGTGGTTGG is reported. The G-quadruplex structure of this thrombin-binding aptamer (TBA), is stabilized by single modifications (T -> 2{\textquoteright}-OMe-U), depending on the position of the modification. The structural stability also renders significantly increased inhibition of thrombin-induced fibrin polymerization, a process closely associated with blood-clotting. (C) 2018 Elsevier Ltd. All rights reserved.},
	doi = {10.1016/j.bmcl.2018.04.027},
	author = {Awachat, Ragini and Wagh, Atish A. and Aher, Manisha and Fernandes, Moneesha and Kumar, Vaijayanti A.}
}
@article {46999,
	title = {Methoxymethyl threofuranosyl thymidine (4 {\textquoteleft}-MOM-TNA-T) at the T7 position of the thrombin-binding aptamer boosts anticoagulation activity, thermal stability, and nuclease resistance},
	journal = {ACS Omega},
	volume = {5},
	year = {2020},
	month = {JAN },
	pages = {498-506},
	type = {Article},
	abstract = {<p>The synthesis of 4{\textquoteright}-methoxymethyl threofuranosyl (4{\textquoteright}-MOM-TNA) thymidine and derived oligomers of the G-rich thrombin-binding aptameric (TBA) sequence is reported. The G-quadruplex stability, anticoagulation activity, and the enzymatic stability of these oligomers bearing the 2{\textquoteright}-3{\textquoteright}-phosphodiester backbone as single substitutions in the loop regions are studied. Amongst all the oligomers, TBA-7T bearing the 4{\textquoteright}-MOM-TNA unit at the T7 position formed a quadruplex with the highest thermal stability. It also resulted in enhanced anticlotting activity that allowed a one-third reduction in the dose, relative to TBA. Further, TBA-7T exhibited enhanced nuclease resistance properties to both endo- and exonucleases.</p>
},
	issn = {2470-1343},
	doi = {10.1021/acsomega.9b03042},
	author = {Varada, Manojkumar and Aher, Manisha and Erande, Namrata and Kumar, Vaijayanti A. and Fernandes, Moneesha}
}
